CD45 Antibody - C-terminal region (OAAB11608)

Catalog No: OAAB11608

Size:400ul

The anti-CD45 (C-term) Pab (Cat. #OAAB11608) is used in Western blot to detect CD45 in jurkat cell lysate.

PTPRC is strongly supported by BioGPS gene expression data to be expressed in jurkat

Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of jurkat cells using CD45 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Precautions: CD45 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN Dephosphorylates LYN, and thereby modulates LYN activity (By similarity)
  Cellular Location: Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PTPRC is strongly supported by BioGPS gene expression data to be expressed in jurkat

• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: PTPRC
• Alias Symbols: LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
• NCBI Gene Id: 5788
• Protein Name: Receptor-type tyrosine-protein phosphatase C
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3
• Molecular Weight: 147254 Da