Human CD45RA Antibody : SPRD (OASB01115)

Catalog No: OASB01115

Size:100T

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: F8-11-13
• Isotype: IgG1
• Host: Mouse
• Conjugation: Spectral Red®
• Application: FC
• Additional Information: Description: CD45RA is a 220 kDa exon A-dependent isoform of CD45. It is expressed on 40-50% of CD4+ peripheral T cells, 50% of peripheral CD8+ T cells, and a portion of B lymphocytes and monocytes.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Purified lymphocytes from human lymph nodes
• Concentration: Lot specific
• Specificity: CD45RA
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Purified (UNLB) antibody
  Fluorescein conjugate
  R-phycoerythrin conjugate
  Spectral Red conjugate
  <= 1 ug/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
• Application Info: Flow cytometry, Immunohistochemistry (frozen sections and paraffin sections), Immunoprecipitation
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze!
  - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected.
• Reference: 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford
  2. Trowbridge, I.S., and M.L. Thomas. 1994. Annu. Rev. Immunol. 12:85
  3. Dalchau, R., and J.W. Fabre. 1981. J. Exp. Med. 153:753.

Target Info

• Gene Symbol: PTPRC
• Gene Full Name: Receptor-type tyrosine-protein phosphatase C
• Alias Symbols: LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
• NCBI Gene Id: 5788
• Protein Name: receptor-type tyrosine-protein phosphatase C
• Description of Target: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3
• Nucleotide Accession #: NM_002838.4