PRMT6 Antibody - N-terminal region (OAAB07235)

Catalog No: OAAB07235

Size:400ul

Western blot analysis of PRMT6 (arrow) using rabbit polyclonal PRMT6 Antibody (N-term) (Cat. #OAAB07235). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRMT6 gene.

PRMT6 Antibody (N-term) (Cat. #OAAB07235) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PRMT6 antibody detected the PRMT6 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC, WB
• Additional Information: Precautions: PRMT6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). It thereby acts as a transcription corepressor of various genes such as HOXA2. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to stimulate the polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC)
  Cellular Location: Nucleus.
  Tissue Location: Highly expressed in kidney and testis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PRMT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from the N-terminal region of human PRMT6.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:2000

Target Info

• Gene Symbol: PRMT6
• Alias Symbols: HRMT1L6
• NCBI Gene Id: 55170
• Protein Name: Protein arginine N-methyltransferase 6
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q96LA8
• Protein Accession #: NP_060607.2
• Molecular Weight: 41938 Da