PRMT6 Antibody (OAAB21647)

Catalog No: OAAB21647

Size:100ul

Western blot detection of PRMT6 in Hela and 293T cell lysates using PRMT6 mouse mAb (OAAB21647) (1:1,000 diluted). Predicted band size: 42KDa. Observed band size: 42KDa.

Immunohistochemistry stain of paraffin-embedded human breast cancer using PRMT6 mouse mAb (OAAB21647) (1:200).

Immunocytochemistry stain of Hela using PRMT6 mouse mAb (OAAB21647) (1:300).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC, IHC
• Additional Information: Function: Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting- induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Application Info: WB: 1:1000
  ICC: 1:300
  IHC: 1:100
• Reference: Frankel A.,et al.J. Biol. Chem. 277:3537-3543(2002).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Gregory S.G.,et al.Nature 441:315-321(2006).
  Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
  Bechtel S.,et al.BMC Genomics 8:399-399(2007).
  

Target Info

• Gene Symbol: PRMT6
• Gene Full Name: protein arginine methyltransferase 6
• Alias Symbols: HRMT1L6
• NCBI Gene Id: 55170
• Description of Target: The protein encoded by this gene belongs to the arginine N-methyltransferase family, which catalyze the sequential transfer of methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins, to form methylated arginine derivatives and S-adenosyl-L-homocysteine. This protein can catalyze both, the formation of omega-N monomethylarginine and asymmetrical dimethylarginine, with a strong preference for the latter. It specifically mediates the asymmetric dimethylation of Arg2 of histone H3, and the methylated form represents a specific tag for epigenetic transcriptional repression. This protein also forms a complex with, and methylates DNA polymerase beta, resulting in stimulation of polymerase activity by enhancing DNA binding and processivity.
• Uniprot ID: Q96LA8
• Protein Accession #: NP_060607.2
• Nucleotide Accession #: NM_018137.2
• Molecular Weight: 42 kDa