Polyclonal Anti-MAP2K3 (OABB00335)

Catalog No: OABB00335

Size:100UG

Lane 1: Rat Spleen Tissue Lysate Lane 2: Rat Thymus Tissue Lysate Lane 3: Rat Skeletal Muscle Tissue Lysate Lane 4: Rat Kidney Tissue Lysate Lane 5: MCF-7 Cell Lysate Lane 6: Hela Cell Lane7: Raji Cell Lysate Lane 8: CEM Cell Lysate Lane 9: Colo320 Cell Lysate

IHC(P): Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al.(1997) localized the MAP2K3 gene to 17q11.2.
  Background: Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al. (1997) localized the MAP2K3 gene to 17q11.2
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse， Rat: By Heat
  Immunocytochemistry/Immunofluorescence: 5 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1.Rampoldi L, Zimbello R, Bortoluzzi S, Tiso N, Valle G, Lanfranchi G, Danieli GA (Mar 1998).
  "Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and
  MEKK5". Cytogenet Cell Genet 78 (3-4): 301–3.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 3(MAP2K3) detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: MAP2K3
• Gene Full Name: mitogen-activated protein kinase kinase 3
• Alias Symbols: dual specificity mitogen-activated protein kinase kinase 3;MAP kinase kinase 3;MAPK/ERK kinase 3;MAPKK 3;MAPKK3;MEK 3;MEK3;MKK3;PRKMK3;SAPK kinase 2;SAPKK2;SAPKK-2;stress-activated protein kinase kinase 2.
• NCBI Gene Id: 5606
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• Description of Target: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
• Uniprot ID: P46734
• Molecular Weight: 39318 MW