MAP2K3 Antibody - N-terminal region (ARP42064_P050)

Catalog#: ARP42064_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-135985 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MAP2K3
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Goat: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
• Peptide Sequence: Synthetic peptide located within the following region: SKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEK
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-MAP2K3 (ARP42064_P050) antibody is Catalog # AAP42064 (Previous Catalog # AAPP24543)
• Target Reference: Prickett,T.D. (2007) Mol. Cell. Biol. 27 (12), 4217-4227
• Gene Symbol: MAP2K3
• Official Gene Full Name: Mitogen-activated protein kinase kinase 3
• Alias Symbols: MAPKK3, MEK3, MKK3, PRKMK3, SAPKK2, SAPKK-2
• NCBI Gene Id: 5606
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• Description of Target: MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
• Swissprot Id: P46734-2
• Protein Accession #: NP_002747
• Nucleotide Accession #: NM_002756
• Protein Size (# AA): 318
• Molecular Weight: 36kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express MAP2K3.
• Protein Interactions: UBC; EGFR; MAPK14; MAP3K5; ARRB1; PKN1; ALDOC; APP; LRRK2; MAPK8IP2; MAPK8IP3; MAPK8IP1; SPAG9; MAP3K2; MAP2K3; PPP2R4; MAP3K4; MAP3K3; TINF2; MYC; RPL13; TAOK2; TAOK1; DCTN1; DYRK1B; PLCB2; SMAD7; MAPK12; MAPK3; ELK1; MAP2K6; NPHS1;

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

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Product Protocols: MAP2K3 antibody tested with Human Transfected 293T Cells (ARP42064_P050)

Product Protocols: MAP2K3 antibody tested by IHC with Human Liver Tissue ARP42064_P050