PIK3CA Antibody - C-terminal region (OAAB18601)

Catalog No: OAAB18601

Size:400ul

Western blot analysis of anti-PI3KCA Pab (Cat. #OAAB18601) in HeLa cell lysate.
PI3KCA (arrow) was detected using purified Pab.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Dilution: 1:1000

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BC = breast carcinoma; HC = hepatocarcinoma.
Dilution: 1:50~100

PIK3CA Antibody (C-term) (Cat. #OAAB18601) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Dilution: 1:10~50

Product Info

• Predicted Species Reactivity: Cow
• Product Format: Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1050 amino acids from the C-terminal region of human PI3KCA.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Cow
• Application Info: WB: 1:1000
  FC: 1:10~50
  IHC-P: 1:50~100
• Reference: Tiwari, S. Nat Immunol. August; 10(8): 907?17 (2009).
  Ballou, L.M., et al., J. Biol. Chem. 278(26):23472-23479 (2003).
  Singh, B., et al., Genes Dev. 16(8):984-993 (2002).
  Shayesteh, L., et al., Nat. Genet. 21(1):99-102 (1999).
  Volinia, S., et al., Genomics 24(3):472-477 (1994).
  Hiles, I.D., et al., Cell 70(3):419-429 (1992).

Target Info

• Gene Symbol: PIK3CA
• Alias Symbols: MCM, CWS5, MCAP, PI3K, CLAPO, CLOVE, MCMTC, PI3K-alpha, p110-alpha
• NCBI Gene Id: 5290
• Description of Target: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. PI3KCA has been found to be oncogenic and has been implicated in cervical cancers.
• Uniprot ID: P42336, P32871
• Protein Accession #: NP_006209.2
• Molecular Weight: 124 kDa