| Product Number |
OAAB18601 |
| Product Page |
www.avivasysbio.com/pik3ca-antibody-c-terminal-oaab18601.html |
| Name |
PIK3CA Antibody - C-terminal region (OAAB18601) |
| Molecular Weight |
124 kDa |
| Isotype |
Rabbit Ig |
| NCBI Gene Id |
5290 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Alias Symbols |
MCM, CWS5, MCAP, PI3K, CLAPO, CLOVE, MCMTC, PI3K-alpha, p110-alpha |
| Product Format |
Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Reference |
Tiwari, S. Nat Immunol. August; 10(8): 907?17 (2009).
Ballou, L.M., et al., J. Biol. Chem. 278(26):23472-23479 (2003).
Singh, B., et al., Genes Dev. 16(8):984-993 (2002).
Shayesteh, L., et al., Nat. Genet. 21(1):99-102 (1999).
Volinia, S., et al., Genomics 24(3):472-477 (1994).
Hiles, I.D., et al., Cell 70(3):419-429 (1992). |
| Description of Target |
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. PI3KCA has been found to be oncogenic and has been implicated in cervical cancers. |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for OAAB18601 |
| Application Info |
WB: 1:1000
FC: 1:10~50
IHC-P: 1:50~100 |
| Immunogen |
This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1050 amino acids from the C-terminal region of human PI3KCA. |
| Uniprot ID |
P42336, P32871 |
| Protein Accession # |
NP_006209.2 |
| Purification |
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Gene Symbol |
PIK3CA |
| Predicted Species Reactivity |
Cow |
| Application |
FC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow |
| Image 1 | Hela cells
 | PIK3CA Antibody (C-term) (Cat. #OAAB18601) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Dilution: 1:10~50 |
| | Image 2 | Human cancer tissue
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
BC = breast carcinoma; HC = hepatocarcinoma.
Dilution: 1:50~100 |
| | Image 3 | HeLa cell lysate
 | Western blot analysis of anti-PI3KCA Pab (Cat. #OAAB18601) in HeLa cell lysate.
PI3KCA (arrow) was detected using purified Pab.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Dilution: 1:1000 |
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