PHGDH Antibody - middle region (ARP54801_P050)

Catalog No: ARP54801_P050

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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Rabbit Anti-PHGDH antibody
Catalog Number: ARP54801
Formalin Fixed Paraffin Embedded Tissue: Human Pancreas
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

Rabbit Anti-PHGDH antibody
Catalog Number: ARP54801
Formalin Fixed Paraffin Embedded Tissue: Human Prostate
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PHGDH
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 92%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
• Peptide Sequence: Synthetic peptide located within the following region: CAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQF
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PHGDH (ARP54801_P050) antibody is Catalog # AAP54801 (Previous Catalog # AAPP31605)
• Sample Type Confirmation:

  PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T

Target Info

• Reference: Tsang,H.T., (2006) Genomics 88 (3), 333-346
• Gene Symbol: PHGDH
• Gene Full Name: Phosphoglycerate dehydrogenase
• Alias Symbols: NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113
• NCBI Gene Id: 26227
• Protein Name: D-3-phosphoglycerate dehydrogenase
• Description of Target: 3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM].
• Uniprot ID: O43175
• Protein Accession #: NP_006614
• Nucleotide Accession #: NM_006623
• Protein Size (# AA): 533
• Molecular Weight: 57 kDa
• Protein Interactions: HUWE1; UBC; FUS; SUMO2; ITSN1; ROM1; RPAP3; EPN1; SUMO1; NEDD8; MDM2; SUZ12; RNF2; EZH2; BMI1; FBXO6; TARDBP; EGFR; UBD; CLN5; CLN3; CDK2; PIAS4; FN1; TRAF3IP1; ECT2; MDC1; UQCC3; VASN; TRIM55; SMU1; TRA2A; ADNP; NCSTN; U2AF2; TCERG1; UTP14A; SRSF10; SAP1