| Product Number |
ARP54801_P050 |
| Product Page |
www.avivasysbio.com/phgdh-antibody-middle-region-arp54801-p050.html |
| Name |
PHGDH Antibody - middle region (ARP54801_P050) |
| Protein Size (# AA) |
533 amino acids |
| Molecular Weight |
57 kDa |
| NCBI Gene Id |
26227 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Phosphoglycerate dehydrogenase |
| Alias Symbols |
NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113 |
| Peptide Sequence |
Synthetic peptide located within the following region: CAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQF |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Tsang,H.T., (2006) Genomics 88 (3), 333-346 |
| Description of Target |
3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]. |
| Protein Interactions |
HUWE1; UBC; FUS; SUMO2; ITSN1; ROM1; RPAP3; EPN1; SUMO1; NEDD8; MDM2; SUZ12; RNF2; EZH2; BMI1; FBXO6; TARDBP; EGFR; UBD; CLN5; CLN3; CDK2; PIAS4; FN1; TRAF3IP1; ECT2; MDC1; UQCC3; VASN; TRIM55; SMU1; TRA2A; ADNP; NCSTN; U2AF2; TCERG1; UTP14A; SRSF10; SAP1 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PHGDH (ARP54801_P050) antibody |
| Blocking Peptide |
For anti-PHGDH (ARP54801_P050) antibody is Catalog # AAP54801 (Previous Catalog # AAPP31605) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PHGDH |
| Uniprot ID |
O43175 |
| Protein Name |
D-3-phosphoglycerate dehydrogenase |
| Sample Type Confirmation |
PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T |
| Protein Accession # |
NP_006614 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006623 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
PHGDH |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 92%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79% |
| Image 1 | Human Pancreas
 | Rabbit Anti-PHGDH antibody
Catalog Number: ARP54801
Formalin Fixed Paraffin Embedded Tissue: Human Pancreas
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
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| Image 2 | Human Prostate
 | Rabbit Anti-PHGDH antibody
Catalog Number: ARP54801
Formalin Fixed Paraffin Embedded Tissue: Human Prostate
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
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| Image 3 |
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
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