PHGDH Antibody - middle region (ARP54802_P050)

Catalog No: ARP54802_P050

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WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: 293T cell lysatePHGDH is supported by BioGPS gene expression data to be expressed in HEK293T

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PHGDH
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Dog: 92%; Horse: 83%; Human: 100%; Mouse: 100%; Pig: 86%; Rabbit: 93%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: SLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPA
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PHGDH (ARP54802_P050) antibody is Catalog # AAP54802 (Previous Catalog # AAPP31606)
• Sample Type Confirmation:

  PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T

• Reference: Tsang,H.T., (2006) Genomics 88 (3), 333-346

Target Info

• Gene Symbol: PHGDH
• Gene Full Name: Phosphoglycerate dehydrogenase
• Alias Symbols: NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113
• NCBI Gene Id: 26227
• Protein Name: D-3-phosphoglycerate dehydrogenase
• Description of Target: 3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM].
• Uniprot ID: O08651
• Protein Accession #: NP_006614
• Nucleotide Accession #: NM_006623
• Protein Size (# AA): 533
• Molecular Weight: 57kDa
• Protein Interactions: HUWE1; UBC; FUS; SUMO2; ITSN1; ROM1; RPAP3; EPN1; SUMO1; NEDD8; MDM2; SUZ12; RNF2; EZH2; BMI1; FBXO6; TARDBP; EGFR; UBD; CLN5; CLN3; CDK2; PIAS4; FN1; TRAF3IP1; ECT2; MDC1; UQCC3; VASN; TRIM55; SMU1; TRA2A; ADNP; NCSTN; U2AF2; TCERG1; UTP14A; SRSF10; SAP1