Catalog No: OKEH03069
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PCCB ELISA Kit (Mouse) (OKEH03069) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pccb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pccb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pccb captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.8% (n = 20)
Mean Inter-assay CV%: <= 8.6% (n = 20)
ELISA Kit Component
Anti-Pccb Microplate96 Wells (12 x 8 Well strips)
Pccb Lyophilized Standard2
100X Biotinylated Pccb Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.164 ng/mL
SpecificityNatural and recombinant Mouse Propionyl-CoA carboxylase beta chain, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPccb
Gene Full Namepropionyl Coenzyme A carboxylase, beta polypeptide
Alias Symbols1300012P06Rik;AI314687;PCCase subunit beta;propanoyl-CoA:carbon dioxide ligase subunit beta;propionyl-CoA carboxylase beta chain, mitochondrial;R74805.
NCBI Gene Id66904
Protein NameQ99MN9
Description of TargetPropionyl-CoA carboxylase beta chain, mitochondrial
Uniprot ID
Protein Accession #NP_001298078.1
Nucleotide Accession #NM_001311149.1
Protein Size (# AA)541
Molecular Weight58 kDa
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