Catalog No: OKEH00338
Size:96T
Price: $525.00
SKU
OKEH00338
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PCCB ELISA Kit (Human) : 96 Wells (OKEH00338)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PCCB ELISA Kit (Human) (OKEH00338) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PCCB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for PCCB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PCCB captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 9.1% (n = 20)
ELISA Kit Component
ComponentAmount
PCCB Microplate96 Wells (12 x 8 Well strips)
PCCB Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PCCB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Propionyl-CoA carboxylase beta chain, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPCCB
Gene Full Namepropionyl-CoA carboxylase subunit beta
Alias SymbolsPCCase subunit beta;propanoyl-CoA:carbon dioxide ligase subunit beta;propionyl CoA carboxylase, beta polypeptide;propionyl Coenzyme A carboxylase, beta polypeptide;propionyl-CoA carboxylase beta chain, mitochondrial;propionyl-CoA carboxylase beta subunit.
NCBI Gene Id5096
Protein NameP05166
Description of TargetPropionyl-CoA carboxylase beta chain, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/P05166
Protein Accession #NP_000523.2
Nucleotide Accession #NM_000532.4
Protein Size (# AA)539
Molecular Weight58 kDa
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