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Catalog No: OKIA00178
Size:1x96WELLPLATE
Price: $577.00
SKU
OKIA00178
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration80 Minutes
ELISA Kit PrincipleAviva Systems Biology NGAL ELISA Kit (Mouse) (OKIA00178) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Neutrophil gelatinase-associated lipocalin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Neutrophil gelatinase-associated lipocalin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Neutrophil gelatinase-associated lipocalin captured in the well.
ELISA Kit Range0.125 ng/ml - 4 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Mouse NGAL Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Mouse NGAL Calibrator1
Additional InformationIntended Use: The Lipocalin-2/NGAL test kits are highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lipocalin-2/NGAL in biological samples of mice.
::REAGENTS
(Quantities sufficient for 96 determinations)
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 L of affinity purified anti-Mouse Lipocalin-2/NGAL antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-Mouse Lipocalin-2/NGAL ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse Lipocalin-2/NGAL.
7. Mouse Lipocalin-2/NGAL CALIBRATOR
One vial containing a Lyophilized Mouse Lipocalin-2/NGAL calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.063 ng/ml
Predicted Homology Based on Immunogen SequenceMouse
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLCN2
Gene Full Namelipocalin 2
Alias SymbolsN, 24p, NRL, 24p3, Sip24, AW212229
NCBI Gene Id16819
Protein NameNeutrophil gelatinase-associated lipocalin
Description of TargetIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Uniprot IDP11672
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