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Datasheets/Manuals | Printable datasheet for OAAB07817 |
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Predicted Species Reactivity | Human |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IF, FC |
Additional Information | Precautions: MSH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
:: | Function: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis |
:: | Cellular Location: Nucleus (Potential). Tissue Location: Ubiquitously expressed. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This MSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the Central region of human MSH2. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Sample Type Confirmation | MSH2 is strongly supported by BioGPS gene expression data to be expressed in K562 |
Application Info | WB: 1:1000 IF: 1:10~50 FC: 1:10~50 |
Gene Symbol | MSH2 |
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Alias Symbols | FCC1, COCA1, HNPCC, LCFS2, hMSH2, HNPCC1, MMRCS2 |
NCBI Gene Id | 4436 |
Protein Name | DNA mismatch repair protein Msh2 |
Description of Target | Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | P43246 |
Protein Accession # | NP_000242.1 |
Molecular Weight | 104743 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MSH2 Antibody - middle region (OAAB07817)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "MSH2 Antibody - middle region (OAAB07817)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "MSH2 Antibody - middle region (OAAB07817)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MSH2 Antibody - middle region (OAAB07817)"?
This target may also be called "FCC1, COCA1, HNPCC, LCFS2, hMSH2, HNPCC1, MMRCS2" in publications.
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What is the shipping cost for "MSH2 Antibody - middle region (OAAB07817)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MSH2 Antibody - middle region (OAAB07817)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MSH2 Antibody - middle region (OAAB07817)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "104743 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MSH2 Antibody - middle region (OAAB07817)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MSH2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MSH2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MSH2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MSH2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MSH2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MSH2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.