MSH2 Antibody (OAEC04258)

Catalog No: OAEC04258

Size:100 ug/100 ul

Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Ascitic fluid containing 0.03% sodium azide
• Clonality: Monoclonal
• Clone: 1B3; 3A2B8C
• Isotype: IgG1
• Host: Mouse
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of human MS H2 expres sed in EColi.
• Concentration: 1 mg/ml
• Application Info: WB: 1/500 - 1/2000
  IHC: 1/200 - 1/1000
  IF: 1/200 - 1/1000
  ELISA: 1/10000
  

Target Info

• Gene Symbol: MSH2
• Gene Full Name: mutS homolog 2
• Alias Symbols: FCC1, COCA1, HNPCC, LCFS2, hMSH2, HNPCC1, MMRCS2
• NCBI Gene Id: 4436
• Description of Target: MS H2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MS H2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MS H2 gene contribute to the development of sporadic colorectal carcinoma. MS HS mutations are responsible for 50% of inherited non-polypos is colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in D NA repair pathways have been related to human carcinogenesis. MS H-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
• Uniprot ID: P43246
• Molecular Weight: 105 kDa