Human CD14 Antibody : APC/CY5.5 (OASB01062)

Catalog No: OASB01062

Size:100Tests

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cynomolgus, Canine, Hooded Seal
• Product Format: Liquid100 tests/mL of PBS, sodium azide, and a stabilizing agent.
• Clonality: Monoclonal
• Clone: 61D3
• Isotype: Mouse (BALB/c) IgG_1k
• Host: Mouse
• Conjugation: APC: Allophycocyanin, CY5.5: Cyanine 5.5
• Application: FC
• Reconstitution and Storage: Store at 2-8C. DO NOT FREEZE!
• Immunogen: Human peripheral monocytes
• Specificity: CD14; Mr 55 kDa
• Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Application Info: FC: 10 uL/10⁶ cells. For FC, the suggested use of these reagents is in a final volume of 100 uL. Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.
• Other Applications Data: Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
• Reference: 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford
  2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD14 Section, Academic Press, New York, p. 169
  3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753
  4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275.

Target Info

• Gene Symbol: CD14
• Gene Full Name: Monocyte differentiation antigen CD14
• NCBI Gene Id: 929; 102129342; 607076
• Protein Name: monocyte differentiation antigen CD14
• Description of Target: CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
• Uniprot ID: P08571, B3Y6B7, A9QU57
• Protein Accession #: NP_000582.1
• Nucleotide Accession #: NM_000591.3
• Molecular Weight: 55 kDa