Size:100 ug
$299.00
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SKU
OAEB02354
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CD14 Antibody (OAEB02354)

Catalog#: OAEB02354
Domestic: within 1 week delivery International: 1 week
This product is Genway GWB-2687EA
More Information
Tested Species Reactivity Human
Predicted Species Reactivity Human
Product Format Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality Polyclonal
Host Goat
Application WB
Reconstitution and Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Replacement Item This antibody may replace item sc-112351, HPA001887, HPA002127
Immunogen Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Complete computational species homology data CD14 Antibody (OAEB02354)
Concentration 0.5 mg/ml
Datasheets/Manuals Printable datasheet for anti-CD14 antibody - OAEB02354
Application Info Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 55 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation is 1 hour at room temperature.
Target Reference * Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.; The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.; Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.; Pubmed 15670766
Publications

Gruber, HE; Riley, FE; Hoelscher, GL; Ingram, JA; Bullock, L; Hanley, EN; Human annulus progenitor cells: Analyses of this viable endogenous cell population. 34, 1351-60 (2016). WB, IHC, Human 27249627

Gene Symbol CD14
Alias Symbols CD14, CD14 antigen, HGNC:1628
NCBI Gene Id 929
Protein Name Monocyte differentiation antigen CD14
Swissprot Id P08571
Protein Accession # NP_000582.1
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CD14.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CD14.
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