CD14 Antibody (OAEB02354)

Catalog No: OAEB02354 (Formerly GWB-2687EA)

Size:100ug

OAEB02354 (1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02354(4ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Concentration: 0.5 mg/ml
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 55 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation is 1 hour at room temperature.
• Reference: * Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.; The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.; Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.; Pubmed 15670766
• Publications:

  Human annulus progenitor cells: Analyses of this viable endogenous cell population. J. Orthop. Res. 34, 1351-60 (2016). 27249627

Target Info

• Gene Symbol: CD14
• Alias Symbols: CD14, CD14 antigen, HGNC:1628
• NCBI Gene Id: 929
• Protein Name: Monocyte differentiation antigen CD14
• Uniprot ID: P08571
• Protein Accession #: NP_000582.1