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CD14 Antibody (OAEB02354)

100 ug
$289.00
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Gene Symbol:
CD14
NCBI Gene Id:
929
Protein Name:
Monocyte differentiation antigen CD14
Swissprot Id:
P08571
Protein Accession #:
NP_000582.1
Alias Symbols:
CD14, CD14 antigen, HGNC:1628
Replacement Item:
This antibody may replace item sc-112351, HPA001887, HPA002127
Host:
Goat
Clonality:
Polyclonal
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application:
WB, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD14.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD14.
Immunogen:
Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Predicted Homology Based on Immunogen Sequence:
Guinea Pig: 76%; Human: 100%
Complete computational species homology data:
CD14 Antibody (OAEB02354)
Peptide Sequence:
C-KRVDADADPRQYAD
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-CD14 antibody - OAEB02354
Application Info:
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Spleen shows strong clustered staining in some splenocytes. Recommended concentration, 4-6ug/ml.
Target Reference:
* Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.; The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.; Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.; Pubmed 15670766
Publications:

Gruber, HE; Riley, FE; Hoelscher, GL; Ingram, JA; Bullock, L; Hanley, EN; Human annulus progenitor cells: Analyses of this viable endogenous cell population. 34, 1351-60 (2016). WB, IHC, Human 27249627

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