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CD14 Antibody (OAEB02354)

100 ug
$349.00
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Gene Symbol:
CD14
NCBI Gene Id:
929
Protein Name:
Monocyte differentiation antigen CD14
Swissprot Id:
P08571
Protein Accession #:
NP_000582.1
Alias Symbols:
CD14, CD14 antigen, HGNC:1628
Replacement Item:
This antibody may replace item sc-112351, HPA001887, HPA002127
Host:
Goat
Clonality:
Polyclonal
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application:
WB, P-ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD14.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD14.
Immunogen:
Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Predicted Species Reactivity:
Human
Tested Species Reactivity:
Human
Complete computational species homology data:
CD14 Antibody (OAEB02354)
Product Format:
Liquid Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
0.5 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-CD14 antibody - OAEB02354
Application Info:
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Approx 55 kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) lysates (calculated MW of 40.1kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15 kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation is 1 hour at room temperature.
Target Reference:
* Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.; The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.; Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.; Pubmed 15670766
Publications:

Gruber, HE; Riley, FE; Hoelscher, GL; Ingram, JA; Bullock, L; Hanley, EN; Human annulus progenitor cells: Analyses of this viable endogenous cell population. 34, 1351-60 (2016). WB, IHC, Human 27249627

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