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Datasheets/Manuals | Printable datasheet for Human CD14 Antibody : SPRD (OASB01058) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Clonality | Monoclonal |
Clone | 61D3 |
Isotype | IgG1 |
Host | Mouse |
Conjugation | Spectral Red® |
Application | FC |
Additional Information | Description: CD14 is a 55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells, and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. |
Reconstitution and Storage | Store at 2-8C |
Immunogen | Human peripheral monocytes |
Concentration | Lot specific |
Specificity | CD14 |
Characterization | To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate PE/CY5.5 and PE/CY7 conjugates APC/CY5.5 and APC/CY7 conjugates AF488 and AF647 conjugates <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells 10 uL/106 cells |
Application Info | Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation |
Other Applications Data | Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need. |
Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC), Alexa Fluor 488 (AF488), and Alexa Fluor 647 (AF647) conjugates are suppliedas 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- PE conjugate and allophycocyanin conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3and a stabilizing agent; store at 2- 8 C - The low endotoxin/azide- free antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS;aliquot and freeze upon receipt - The Spectral Red (SPRD), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), R- phycoerythrin- Cyanine 7(R- PE- CY 7), Allophycocyanin- Cyanine 5.5 (APC- CY 5.5), and Allophycocyanin- Cyanine 7 (APCCY 7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at2- 8 C. Do not freeze! - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected. |
Reference | 1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD14 Section, Academic Press, New York, p. 169 3. Hogg, N., S. MacDonald, M. Slusarenko, and P.C. Beverley. 1984. Immunol. 53:753 4. Stocks, S.C., M. Albrechtsen, and M.A. Kerr. 1990. Biochem. J. 268:275. |
Gene Symbol | CD14 |
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Gene Full Name | Monocyte differentiation antigen CD14 |
NCBI Gene Id | 929; 102129342; 607076 |
Protein Name | monocyte differentiation antigen CD14 |
Description of Target | The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. |
Uniprot ID | P08571, B3Y6B7, A9QU57 |
Protein Accession # | NP_000582.1 |
Nucleotide Accession # | NM_000591.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Human CD14 Antibody : SPRD (OASB01058)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Human CD14 Antibody : SPRD (OASB01058)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Human CD14 Antibody : SPRD (OASB01058)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Human CD14 Antibody : SPRD (OASB01058)"?
This target may also be called "" in publications.
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What is the shipping cost for "Human CD14 Antibody : SPRD (OASB01058)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Human CD14 Antibody : SPRD (OASB01058)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Human CD14 Antibody : SPRD (OASB01058)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Human CD14 Antibody : SPRD (OASB01058)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD14"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD14"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD14"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD14"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD14"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD14"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.