MICB Antibody (OASA03118)

Catalog No: OASA03118 (Formerly GWB-Q01146)

Size:200UG

MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells.
MICA is principally expressed on intestinal epithelium, and several epithelial tumors. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection.
Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: 6D4
• Isotype: IgG2a
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Frozen|Immunoprecipitation
• Additional Information: Fusion Partners: Spleen cells from immunised RBF/DnJ mice were fused with cells of the P3 mouse myeloma cell line.
• Reconstitution and Storage: -20°C
• Immunogen: MICA transfected C1R cells.
• Purification: Protein A Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: MICA/MICB
• Application Info: FC: 1/50 - 1/200. Use 10 uL of the suggested working dilution to label 1x10⁶ cells in 100 uL.
  IHC-F: 1/100
• Reference: 1. Groh, V.et al. (1998) Recognition of stress-induced MHC molecules by intestinal epithelial gamma delta T cells. Science. 279: 1737-1740.
  2. Bauer, S. et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285: 727-729.
  3. Groh, V. et al. (1999) Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc. Natl. Acad. Sci. USA. 96: 6879-6884.
  4. Groh, V. et al. (2001) Costimulation of CD8 alpha beta T cels by NKG2D via engagement by MIC induced on virus-infected cells. Nat Immunol. 2: 255-60.
  5. Das, H. et al. (2004) Mechanisms of V delta 1 T cell activation by microbial components. J. Immunol. 172: 6578-6586.
  6. Jinushi, M. et al. (2003) Critical role of MHC class I - related chain A and B expression on IFN - alpha - stimulated dendritic cells in NK cell activation: Impairment in chronic hepatitis C virus infection. J. Immunol. 170: 1249-1256.
  

Target Info

• Gene Symbol: MICB
• Alias Symbols: PERB11.2
• NCBI Gene Id: 4277
• Protein Name: MHC class I polypeptide-related sequence B
• Uniprot ID: Q29980
• Protein Accession #: NP_005922.2
• Protein Size (# AA): 383