MED7 Antibody - middle region (OAAB08203)

Catalog No: OAAB08203

Size:400ul

MED7 Antibody (Center) (Cat. #OAAB08203) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MED7 antibody detected the MED7 protein (arrow).

MED7 is supported by BioGPS gene expression data to be expressed in Hela

MED7 Antibody (Center) (Cat. #OAAB08203) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse, Cow, Pig
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Precautions: MED7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
  Cellular Location: Nucleus.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MED7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human MED7.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Pig
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  MED7 is supported by BioGPS gene expression data to be expressed in Hela

• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: MED7
• Alias Symbols: ARC34, CRSP9, CRSP33
• NCBI Gene Id: 9443
• Protein Name: Mediator of RNA polymerase II transcription subunit 7
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O43513
• Protein Accession #: NP_001094286.1
• Molecular Weight: 27245 Da