MCM7 Polyclonal Antibody (OABB00743)

Catalog No: OABB00743

Size:100UG

IHC(P):Human Lung Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Hamster|Human
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al.(2008) found that MCM7 and the precursors of microRNAs(miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
  Background: MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al. (2008) found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunocytochemistry: 0.5-1 ug/ml: Human: Immunocytochemistry/Immunofluorescence: 5 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of
  replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007.
  2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a
  product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68:
  226-230, 1995.
  3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D.,
  Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G.,
  Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle
  arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM7(MCM7) detection. Tested with WB, IHC-P, ICC, ICC/IF, FCM in Human.

Target Info

• Gene Symbol: MCM7
• Gene Full Name: minichromosome maintenance complex component 7
• Alias Symbols: CDC47;CDC47 homolog;DNA replication licensing factor MCM7;homolog of S. cerevisiae Cdc47;MCM2;minichromosome maintenance deficient 7;P1.1-MCM3;P1CDC47;P85MCM;PNAS146;PPP1R104;protein phosphatase 1, regulatory subunit 104.
• NCBI Gene Id: 4176
• Protein Name: DNA replication licensing factor MCM7
• Description of Target: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
• Uniprot ID: P33993
• Molecular Weight: 81308 MW