MCM2 Polyclonal Antibody (OABB00603)

Catalog No: OABB00603

Size:100UG

Lane1: SW620 Cell Lysate
Lane2:PANC Cell Lysate Cancer Tissue Cancer Tissue
Lane3: Jurkat Cell Lysate
Lane4:Hela Cell Lysate

IHC(P):Human Intestinal

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Hamster|Human|Mouse
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2),also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
  Background: MCM2(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2) ， also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunocytochemistry : 0.5-1 ug/ml: Human
  Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. Blow, J. J., Laskey, R. A. A role for the nuclear envelope in controlling DNA replication within the cell
  cycle. Nature 332: 546-548, 1988.
  2. Burkhart, R., Schulte, D., Hu, D., Musahl, C., Gohring, F., Knippers, R. Interactions of human nuclear
  proteins P1Mcm3 and P1Cdc46. Europ. J. Biochem. 228: 431-438, 1995.
  3. Todorov IT, Lavigne J, Sakr F, Kaneva R, Foisy S, Bibor-Hardy V (Jul 1991). "Nuclear matrix protein
  mitotin messenger RNA is expressed at constant levels during the cell cycle". Biochem Biophys Res
  Commun 177 (1): 395–400
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC, ICC/IF, FCM in Human;Mouse.

Target Info

• Gene Symbol: MCM2
• Gene Full Name: minichromosome maintenance complex component 2
• Alias Symbols: BM28;CCNL1;cdc19;CDCL1;cell devision cycle-like 1;cyclin-like 1;D3S3194;DFNA70;DNA replication licensing factor MCM2;minichromosome maintenance deficient 2 (mitotin);minichromosome maintenance protein 2 homolog;MITOTIN;nuclear protein BM28.
• NCBI Gene Id: 4171
• Protein Name: DNA replication licensing factor MCM2
• Description of Target: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
• Uniprot ID: P49736
• Molecular Weight: 101896 MW