LCN2 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03827)

Catalog No: OKCD03827

Size:96WELLS

Mouse LCN2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-104%	87-95%	96-103%	80-91%
  EDTA Plasma (n=5)	80-101%	92-101%	83-97%	80-98%
  Heparin Plasma (n=5)	78-104%	91-105%	84-91%	97-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil gelatinase-associated lipocalin (NGAL) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-101	96
  EDTA Plasma (n=5)	82-91	85
  Heparin Plasma (n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Lcn2 Microplate: 96 Wells (12 x 8 Well strips)
  • Lcn2 Lyophilized Standard: 2
  • 100X Lcn2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0274 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Lcn2
• Gene Full Name: lipocalin 2
• Alias Symbols: 24p;24p3;AW212229;Lipocalin-2;N;neu-related lipocalin;neutrophil gelatinase-associated lipocalin;NRL;oncogene 24p3;p25;secreted inducible protein 24;siderocalin LCN2;Sip24;SV-40-induced 24P3 protein.
• NCBI Gene Id: 16819
• Protein Name: Neutrophil gelatinase-associated lipocalin
• Description of Target: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
• Uniprot ID: P11672
• Protein Accession #: NP_032517
• Nucleotide Accession #: NM_008491