Catalog No: OPPA02410 (Formerly GWB-BSP225)
Size:2UG
Price: $75.00
SKU
OPPA02410
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA02410 |
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Predicted Species Reactivity | Human |
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Product Format | Formulation: The LCAT protein was lyophilized from 0.4?m filtered solution at a concentration of 0.5mg/ml containing 20mM Tris buffer, and 50mM NaCl, pH 7.5. Physical appearance: Filtered White lyophilized (freeze-dried) powder. |
Host | Human Embryonic Kidney 293 cells |
Additional Information | Solubility: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. LCAST HEK is not sterile |
:: | Product Introduction: LCAT is an extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. LCAT is a essential enzyme in the extracellular metabolism of plasma lipoproteins. Product Description: LCAT Human Recombinant produced in HEK is a single, glycosylated, polypeptide chain containing 429 amino acids (25-440) which includes a 13 amino acid Flag Tag fused at N-terminus and having a total molecular mass of 48.5 kDa. LCAT Human Recombinant is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized LCAT although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution LCAT should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Peptide Sequence | HVDYKDDDDK PAGFWLLNVL FPPHTTPKAE LSNHTRPVIL VPGCLGNQLE AKLDKPDVVN WMCYRKTEDF FTIWLDLNMF LPLGVDCWID NTRVVYNRSS GLVSNAPGVQ IRVPGFGKTY SVEYLDSSKL AGYLHTLVQN LVNNGYVRDE TVRAAPYDWR LEPGQQEEYY RKLAGLVEEM HAAYGKPVFL IGHSLGCLHL LYFLLRQPQA WKDRFIDGFI SLGAPWGGSI KPMLVLASGD NQGIPIMSSI KLKEEQRITT TSPWMFPSRM AWPEDHVFIS TPSFNYTGRD FQRFFADLHF EEGWYMWLQS RDLLAGLPAP GVEVYCLYGV GLPTPRTYIY DHGFPYTDPV GVLYEDGDDT VATRSTELCG LWQGRQPQPV HLLPLHGIQH LNMVFSNLTL EHINAILLGA YRQGPPASPT ASPEPPPPE |
Source | HEK293 Cells |
Gene Symbol | LCAT |
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Alias Symbols | Lecithin-Cholesterol Acyltransferase Human Recombinant, HEK, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT |
NCBI Gene Id | 3931 |
Protein Name | Phosphatidylcholine-sterol acyltransferase |
Description of Target | LCAT Human, HEK |
Uniprot ID | P04180 |
Protein Accession # | NP_000220.1 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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