KCC2 Antibody (OASE00240)

Catalog No: OASE00240

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide.
• Clonality: Monoclonal
• Clone: S1-12
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP
• Additional Information: Cellular Localization: Membrane
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 932-1043 corresponding to rat KCC2
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~140kDa.
• Dilution: WB (1:1000), IHC (1:300), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application Info: WB: 1:1000
  IHC: 1:300
  ICC: 1:100
  IF: 1:100
  Optimal dilutions should be determined by the end user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Publications:

  Pharmaco-resistant Neonatal Seizures: Critical Mechanistic Insights from a Chemoconvulsant Model. Dev Neurobiol. 78, 1117-1130 (2018). 30136373

  TrkB agonists prevent postischemic emergence of refractory neonatal seizures in mice. JCI Insight. 5, NULL (2020). 32427585

• Reference: 1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784.
  2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988.
  3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217.
  4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.
  
• Description:
  View product citations for KCC2 Antibody (OASE00240) on CiteAb

Target Info

• Gene Symbol: KCC2
• Gene Full Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Alias Symbols: Potassium Chloride Cotransporter, Potassium chloride transporter 5, SLC12A5, hKCC2, S12A5, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter, KIAA1176, Electroneutral potassium chloride cotransporter 2, Erythroid K Cl cotransporter 2, Furosemide sensitive K Cl cotransporter, K-Cl cotransporter 2, KCC 2, Neuronal K Cl cotransporter, Solute carrier family 12 (potassium chloride transporter) member 5, Solute carrier family 12 member 5
• NCBI Gene Id: 171373
• Description of Target: KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
• Uniprot ID: Q63633
• Protein Accession #: NP_599190
• Molecular Weight: 140 kDa