ISG15 ELISA Kit (Mouse) (OKCA02472)

Catalog No: OKCA02472

Size:96 Wells

Mouse ISG15 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	106	100-112
  1:2	84	80-88
  1:4	89	84-93
  1:8	98	94-102

• ELISA Kit Principle: Aviva Systems Biology Isg15 ELISA Kit (Mouse) (OKCA02472) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Isg15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Isg15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Isg15 captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	95	90-99
  EDTA Plasma (n=4)	99	94-103

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Isg15 Microplate: 96 Wells (12 x 8 Well strips)
  • Isg15 Lyophilized Standard: 2
  • 100X Biotinylated Isg15 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
• Sensitivity: 0.078 ng/mL
• Publications:

  Modulation of Extracellular ISG15 Signaling by Pathogens and Viral Effector Proteins. Cell Rep. 31, 107772 (2020). 32553163

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for ISG15 ELISA Kit (Mouse) (OKCA02472) on CiteAb

Target Info

• Gene Symbol: ISG15
• Alias Symbols: G1p, G1p2, IP17, Irfp, UCRP, IGI15
• NCBI Gene Id: 100038882
• Protein Name: Ubiquitin-like protein ISG15
• Description of Target: Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response and EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.
• Uniprot ID: Q64339
• Protein Accession #: NP_056598
• Nucleotide Accession #: NM_015783