ISG15 Antibody - N-terminal region (OAAB07754)

Catalog No: OAAB07754

Size:400ul

ISG15 Antibody western blot analysis in Jurkat? K562 and AML-2 cell line lysates treated with a small molecular of 0?10?20?30uM for 24 hours.(Offered friendly by Biyin Cao, Soochow University, Center for Blood Research)

Western blot analysis of ISG15 (arrow) using rabbit polyclonal hISG15-A46 (Cat. #OAAB07754). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2).

ISG15 Antibody (A46) (Cat. #OAAB07754) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ISG15 antibody detected the ISG15 protein (arrow).

ISG15 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC, WB
• Additional Information: Precautions: ISG15 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections
  Cellular Location: Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted
  Tissue Location: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ISG15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human ISG15.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  ISG15 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: IHC-P: 1:50~100
  WB: 1:1000

Target Info

• Gene Symbol: ISG15
• Alias Symbols: G1P2, IP17, UCRP, IFI15, IMD38, hUCRP
• NCBI Gene Id: 9636
• Protein Name: Ubiquitin-like protein ISG15
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P05161
• Protein Accession #: NP_005092.1
• Molecular Weight: 17888 Da