Catalog No: OKCA00271
Size:96 Wells
Price: $600.00
SKU
OKCA00271
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Insulin ELISA Kit (Goat) : 96 Wells (OKCA00271)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityGoat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:195%90-101%
Serum (n=4)1:290%80-94%
Serum (n=4)1:498%90-105%
Serum (n=4)1:890%80-96%
ELISA Kit PrincipleAviva Systems Biology Insulin ELISA Kit (Goat) (OKCA00271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Goat monoclonal antibody specific for Goat Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Goat Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Goat Insulin captured in well.
ELISA Kit Range8 uIU/mL - 140 uIU/mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (μIU/mL)33.7732.48630.44730.91331.65632.302
SD0.020.0220.0190.0270.0260.029
CV%5.6126.2745.8898.2117.6548.369
ELISA Kit Component
ComponentAmount
Anti-Insulin Microplate96 Well (12 x 8 Well Strips)
Insulin Standard5 x 500 uL
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity5 uIU/ml
SpecificityThis assay has high sensitivity and excellent specificity for detection of goat INS. No significant cross-reactivity or interference between goat INS and analogues was observed
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolInsulin
Protein NameInsulin
Description of TargetAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.
Swissprot IdP01319
Protein Size (# AA)51
Molecular Weight6 kDa
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