Product Number |
OKCA00271 |
Product Page |
www.avivasysbio.com/insulin-elisa-kit-goat-96-wells-okca00271.html |
Name |
Insulin ELISA Kit (Goat) : 96 Wells (OKCA00271) |
Protein Size (# AA) |
51 amino acids |
Molecular Weight |
6 kDa |
Description of Target |
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region. |
Reconstitution and Storage |
Store as indicated in product manual. |
Datasheets/Manuals |
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Specificity |
This assay has high sensitivity and excellent specificity for detection of goat INS. No significant cross-reactivity or interference between goat INS and analogues was observed |
Assay Info |
Assay Methodology: Quantitative Sandwich Immunoassay |
Sensitivity |
5 uIU/ml |
Uniprot ID |
P01319 |
Protein Name |
Insulin |
Sample Type |
Serum, Plasma, Tissue homogenates |
ELISA Kit Component |
Component | Amount |
---|
Anti-Insulin Microplate | 96 Well (12 x 8 Well Strips) | Insulin Standard | 5 x 500 uL | HRP-Conjugate | 1 x 6 mL | 20X Wash Buffer | 1 x 15 mL | Substrate A | 1 x 7 mL | Substrate B | 1 x 7 mL | Stop Solution | 1 x 7 mL |
|
ELISA Kit Reproducibility |
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 | n | 20 | 20 | 20 | 20 | 20 | 20 | Mean (?IU/mL) | 33.77 | 32.486 | 30.447 | 30.913 | 31.656 | 32.302 | SD | 0.02 | 0.022 | 0.019 | 0.027 | 0.026 | 0.029 | CV% | 5.612 | 6.274 | 5.889 | 8.211 | 7.654 | 8.369 |
|
ELISA Kit Range |
8 uIU/mL - 140 uIU/mL |
ELISA Kit Duration |
2.5 Hours |
ELISA Kit Principle |
Aviva Systems Biology Insulin ELISA Kit (Goat) (OKCA00271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Goat monoclonal antibody specific for Goat Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Goat Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Goat Insulin captured in well. |
ELISA Kit Linearity |
Sample Type 1 |
---|
Sample Type | Dilution | Average % | Range % |
---|
Serum (n=4) | 1:1 | 95% | 90-101% | Serum (n=4) | 1:2 | 90% | 80-94% | Serum (n=4) | 1:4 | 98% | 90-105% | Serum (n=4) | 1:8 | 90% | 80-96% |
|
ELISA Kit Detection Method |
Colorimetric, OD450 nm |
Gene Symbol |
Insulin |
Predicted Species Reactivity |
Goat |
Image 1 | |
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