Insulin ELISA Kit (Goat) : 96 Wells (OKCA00271)

Data Sheet
 
Product Number OKCA00271
Product Page www.avivasysbio.com/insulin-elisa-kit-goat-96-wells-okca00271.html
Name Insulin ELISA Kit (Goat) : 96 Wells (OKCA00271)
Protein Size (# AA) 51 amino acids
Molecular Weight 6 kDa
Description of Target After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.
Reconstitution and Storage Store as indicated in product manual.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of goat INS. No significant cross-reactivity or interference between goat INS and analogues was observed
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Sensitivity 5 uIU/ml
Uniprot ID P01319
Protein Name Insulin
Sample Type Serum, Plasma, Tissue homogenates
ELISA Kit Component
ComponentAmount
Anti-Insulin Microplate96 Well (12 x 8 Well Strips)
Insulin Standard5 x 500 uL
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020202020
Mean (?IU/mL)33.7732.48630.44730.91331.65632.302
SD0.020.0220.0190.0270.0260.029
CV%5.6126.2745.8898.2117.6548.369
ELISA Kit Range 8 uIU/mL - 140 uIU/mL
ELISA Kit Duration 2.5 Hours
ELISA Kit Principle Aviva Systems Biology Insulin ELISA Kit (Goat) (OKCA00271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Goat monoclonal antibody specific for Goat Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Goat Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Goat Insulin captured in well.
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:195%90-101%
Serum (n=4)1:290%80-94%
Serum (n=4)1:498%90-105%
Serum (n=4)1:890%80-96%
ELISA Kit Detection Method Colorimetric, OD450 nm
Gene Symbol Insulin
Predicted Species Reactivity Goat
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