Catalog No: OKEH00908
Size:96 Wells
Price: $600.00
SKU
OKEH00908
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Insulin AutoAntibody ELISA Kit (Human) (OKEH00908)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00908
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Insulin Autoantibody ELISA Kit (Human) (OKEH00908) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Insulin Autoantibody antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Insulin Autoantibody and incubated. The Insulin Autoantibody found in the sample or standards competes with the biotinylated Insulin Autoantibody for limited binding sites on the immobilized anti-human Insulin Autoantibody antibody. Excess unbound biotinylated Insulin Autoantibody and sample or standard Insulin Autoantibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Insulin Autoantibody captured in the well and inversely proportional to the amount of Insulin Autoantibody which was contained in the sample or standard.
ELISA Kit Range3.12 - 200ng/ml
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Insulin Autoantibody Microplate96 Wells (12 x 8 Well strips)
Insulin Autoantibody Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Insulin Autoantibody-Biotin Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.16 ng/mL
SpecificityNatural and recombinant Human Insulin Autoantibodies
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolInsulin Autoantibody
Alias SymbolsInsulin Autoantibody
Protein NameInsulin Autoantibody
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