Insulin AutoAntibody ELISA Kit (Human) (OKEH00908)

Catalog No: OKEH00908

Size:96T

Human IAA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Insulin Autoantibody ELISA Kit (Human) (OKEH00908) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Insulin Autoantibody antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Insulin Autoantibody and incubated. The Insulin Autoantibody found in the sample or standards competes with the biotinylated Insulin Autoantibody for limited binding sites on the immobilized anti-human Insulin Autoantibody antibody. Excess unbound biotinylated Insulin Autoantibody and sample or standard Insulin Autoantibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Insulin Autoantibody captured in the well and inversely proportional to the amount of Insulin Autoantibody which was contained in the sample or standard.
• ELISA Kit Range: 3.12-200ng/ml
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.2% (n = 20)
• ELISA Kit Component:
  • Anti-Insulin Autoantibody Microplate: 96 Wells (12 x 8 Well strips)
  • Insulin Autoantibody Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Insulin Autoantibody-Biotin Complex: 1 x 60 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.16 ng/mL
• Specificity: Natural and recombinant Human Insulin Autoantibodies
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Insulin Autoantibody
• Alias Symbols: Insulin Autoantibody
• Protein Name: Insulin Autoantibody