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IL6 ELISA Kit (Human) : 96 Wells (OKAA00012-96W)

96 Wells
$370.00
In Stock
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Predicted Species Reactivity:
Human
Application:
ELISA
Kit Detection Method:
Colorimetric, OD450 nm
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IL-9 Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-9 monoclonal antibody
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-6 (8x12)
2. 2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-6 polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
2 pg/ml
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay recognizes both natural and recombinant human IL-6. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-6 sR, IL-6 sR/sgp130, IL-9, IL-10, G-CSF,
Recombinant mouse: IL-6, IL-10, IL-11, IL-12/23 p40,
Recombinant porcine: CNTF, G-CSF, IL-6, OSM,
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Gene Symbol:
IL6
Official Gene Full Name:
interleukin 6
Alias Symbols:
B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFNB2, IFN-beta-2, IL-6, Interferon beta-2, Interleukin-6
NCBI Gene Id:
3569
Protein Name:
Interleukin-6
Description of Target:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
Swissprot Id:
P05231
Protein Accession #:
NP_000591
Nucleotide Accession #:
NM_000600
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL6.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL6.

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