- Predicted Species Reactivity:
- Product Format:
- 1 x 96-Well Plate or 12 x 8-Well Strips
- Kit Detection Method:
- Colorimetric, OD450 nm
- Kit Principle:
- The Aviva Human IL-6 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-6 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IL-6 cytokine while the user-added detection antibodies bind to epitopes on the bound target protein. After incubation and a "sandwich" complex is formed by the capture antibody, the target antigen, and the biotinylated detection antibody, a single wash step is needed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. A peroxidase enzyme is then added to conjugate to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration
- Kit Range:
- 32-2,000 pg/mL
- Kit Component:
|Capture Antibody Coated Microplate||96 Wells (12 x 8)|
|Ready-to-Use Streptavidin-HRP||12 mL|
|Ready-to-Use Substrate||12 mL|
|Stop Solution||12 mL|
|Wash Buffer (15x)||50 mL|
|Protein Standard Diluent||12 mL|
|Sample Diluent||12 mL|
|Detection Antibody Diluent||12 mL|
|Biotin-Labeled Detection Antibody||Lyophilized|
|Protein Standard||Lyophilized (100 ng)|
- Reconstitution and Storage:
- Store as indicated in product manual.
- Official Gene Full Name:
- interleukin 6
- Alias Symbols:
- B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFNB2, IFN-beta-2, IL-6, Interferon beta-2, Interleukin-6
- NCBI Gene Id:
- Protein Name:
- Description of Target:
- This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
- Sample Type:
- Cell lysates, sera and plasma
- 32 pg/mL
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express IL6.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express IL6.
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- The Human IL-6 ELISA is capable of recognizing both recombinant and naturally produced Human IL-6 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: CNTF, CT-1, G-CSF, sIL-6R, IL-11, IL-12, Leptin, OSM
• Murine: CT-1, G-CSF, IL-6, IL-12
• Rat: IL-6