IL-6 ELISA Kit (Human) : 96 Wells (OKAG00041)

Catalog No: OKAG00041 (Formerly GWB-SKR041)

Size:12x8WELLS

IL-6 ELISA Kit (Human) (OKAG00041) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Product Format: 1 x 96-Well Plate or 12 x 8-Well Strips
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric 450 nm
• ELISA Kit Duration: 4.5 hours
• ELISA Kit Principle: The Aviva Human IL-6 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-6 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IL-6 cytokine while the user-added detection antibodies bind to epitopes on the bound target protein. After incubation and a "sandwich" complex is formed by the capture antibody, the target antigen, and the biotinylated detection antibody, a single wash step is needed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. A peroxidase enzyme is then added to conjugate to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration
• ELISA Kit Range: 32-2000 pg/ml
• ELISA Kit Component:
  • Capture Antibody Coated Microplate: 96 Wells (12 x 8)
  • Ready-to-Use Streptavidin-HRP: 12 mL
  • Ready-to-Use Substrate: 12 mL
  • Stop Solution: 12 mL
  • Wash Buffer (15x): 50 mL
  • Protein Standard Diluent: 12 mL
  • Sample Diluent: 12 mL
  • Detection Antibody Diluent: 12 mL
  • Biotin-Labeled Detection Antibody: Lyophilized
  • Protein Standard: Lyophilized (100 ng)
• Reconstitution and Storage: 2°C to 8°C
• Sample Type: Cell lysates, sera and plasma
• Sensitivity: 32 pg/mL
• Specificity: The Human IL-6 ELISA is capable of recognizing both recombinant and naturally produced Human IL-6 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
  • Human: CNTF, CT-1, G-CSF, sIL-6R, IL-11, IL-12, Leptin, OSM
  • Murine: CT-1, G-CSF, IL-6, IL-12
  • Rat: IL-6
• Assay Info: Quantitative Colorimentric Sandwich ELISA

Target Info

• Gene Symbol: IL6
• Gene Full Name: interleukin 6
• Alias Symbols: B-cell differentiation factor;B-cell stimulatory factor 2;BSF2;BSF-2;CDF;CTL differentiation factor;HGF;HSF;hybridoma growth factor;IFNB2;IFN-beta-2;IL-6;interferon beta-2;interleukin BSF-2;interleukin-6.
• NCBI Gene Id: 3569
• Protein Name: P05231
• Description of Target: Interleukin-6
• Uniprot ID: https://www.uniprot.org/uniprot/P05231
• Protein Accession #: NP_000591.1
• Nucleotide Accession #: NM_000600.4