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IL-6 ELISA Kit (Human) : 96 Wells (OKAG00041)

96 Wells
$419.00
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Predicted Species Reactivity:
Human
Product Format:
1 x 96-Well Plate or 12 x 8-Well Strips
Application:
ELISA
Kit Detection Method:
Colorimetric, OD450 nm
Kit Principle:
The Aviva Human IL-6 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hIL-6 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human IL-6 cytokine while the user-added detection antibodies bind to epitopes on the bound target protein. After incubation and a "sandwich" complex is formed by the capture antibody, the target antigen, and the biotinylated detection antibody, a single wash step is needed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. A peroxidase enzyme is then added to conjugate to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration
Kit Range:
32-2,000 pg/mL
Kit Component:
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (100 ng)
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
IL6
Official Gene Full Name:
interleukin 6
Alias Symbols:
B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFNB2, IFN-beta-2, IL-6, Interferon beta-2, Interleukin-6
NCBI Gene Id:
3569
Protein Name:
Interleukin-6
Description of Target:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.
Swissprot Id:
P05231
Protein Accession #:
NP_000591.1
Nucleotide Accession #:
NM_000600.4
Sample Type:
Cell lysates, sera and plasma
Sensitivity:
32 pg/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL6.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL6.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
The Human IL-6 ELISA is capable of recognizing both recombinant and naturally produced Human IL-6 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: CNTF, CT-1, G-CSF, sIL-6R, IL-11, IL-12, Leptin, OSM
• Murine: CT-1, G-CSF, IL-6, IL-12
• Rat: IL-6
Assay Info:
Quantitative Colorimentric Sandwich ELISA

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