HSPB1 Recombinant Protein (Human) (OPED00065)

Catalog No: OPED00065

Size:50 ug

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alpha B-crystallin. It is characterized by a conserved C-terminal alpha -crystallin domain consisting of two anti-parallel beta -sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha , p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Figure 1: SDS-PAGE Analysis - Lane 1: MW Marker; Lane 2: 1μg Hsp27

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid 1X Dulbecco's PBS
• Host: E. Coli
• Application: WB
• Additional Information: Endotoxin Level: < 50 EU/mg purified protein (LAL test)
• Reconstitution and Storage: Store at -80°C. Avoid repeated freeze/thaw cycles.
• Purification: Purified by multi-step chromatography.
• Concentration: Varies by lot. See vial for concentration.
• Purity: ≥ 95% (SDS-PAGE, Western blot)
• Specificity: Immunoreactive with antibody specific for Hsp27
• Application Info: Western blot control.

Target Info

• Gene Symbol: HSPB1
• Gene Full Name: heat shock protein family B (small) member 1
• Alias Symbols: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102
• NCBI Gene Id: 3315
• Protein Name: Heat shock protein beta -1
• Description of Target: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
• Uniprot ID: P04792
• Protein Accession #: NP_001138610.1
• Nucleotide Accession #: NM_001145138.1
• Protein Size (# AA): 205
• Molecular Weight: 27 kDa