HSPB1 Antibody (OAGA01492)

Catalog No: OAGA01492

Size:100 ul

HSP27 antibody detects HSP27 protein by western blot analysis.
A. 30 ug A431 whole cell extract
B. 30 ug HeLa whole cell extract
12 % SDS-PAGE
HSP27 antibody (OAGA01492) dilution: 1:10000

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
OAGA01492 diluted at 1:1000

HSP27 antibody detects HSPB1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
HSP27 antibody (OAGA01492) dilution: 1:500.

HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HSP27 protein stained by HSP27 antibody (OAGA01492) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 ug of HSP27 antibody (OAGA01492).
Western blot analysis was performed using HSP27 antibody (OAGA01492).
EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 1XPBS, 20% glycerol (pH 7) with 0.025% ProClin300 added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: FC, ICC, IF, IHC-FFPE, IP, WB
  Positive Controls: 293T, A431, HeLa, HepG2, HT-29, mouse liver
  Cellular Location: Cytoplasm, Nucleus, Spindle
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography
• Predicted Homology Based on Immunogen Sequence: Dog (86%), Pig (86%), Rhesus Monkey (100%), Bovine (83%)
• Concentration: 0.45 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  IP: 1:100~1000
  WB: 1:500~20000
  FC: Assay-dependent dilution

Target Info

• Gene Symbol: HSPB1
• Gene Full Name: heat shock 27kDa protein 1
• Alias Symbols: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102
• NCBI Gene Id: 3315
• Description of Target: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
• Uniprot ID: P04792
• Molecular Weight: 23 kDa