HSPB1 Antibody - C-terminal region (ARP30177_T100)

Catalog#: ARP30177_T100

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: HSPB1
• Official Gene Full Name: Heat shock 27kDa protein 1
• NCBI Gene Id: 3315
• Protein Name: Heat shock protein beta-1
• Swissprot Id: P04792
• Protein Accession #: NP_001531
• Nucleotide Accession #: NM_001540
• Alias Symbols: CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25, HMN2B, SRP27
• Replacement Item: This antibody may replace item sc-1048 from Santa Cruz Biotechnology.
• Description of Target: HSPB1, like the other heat shock proteins, is part of a complex system of molecular chaperones in epidermal keratinocytes.
• Protein Size (# AA): 205
• Molecular Weight: 23kDa
• Purification: Protein A purified
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express HSPB1.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HSPB1
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: Synthetic peptide located within the following region: SLSPEGTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: PCSK9; HUWE1; UBC; GATA1; MAPKAPK2; AKT1; CRYAB; MAP3K7; SUMO2; HAUS1; CEP57; AARSD1; BAG3; IRS4; HSF1; LCE3A; TGFB1I1; SUMO1; NEDD8; MDM2; ASB6; ASB10; ASB17; WWOX; ASB4; ZBTB1; RPA3; RPA2; RPA1; EZH2; ESR1; SOX2; NPM1; PLG; CS; MAPKAPK3; INS; HSPB1; SGA
• Blocking Peptide: For anti-HSPB1 (ARP30177_T100) antibody is Catalog # AAP30177 (Previous Catalog # AAPS08907)
• Target Reference: Boncinelli,E., (2006) J. Biol. Chem. 281 (12), 8169-8174
• Publications:

  Narjoz, C. et al. Genomic consequences of cytochrome P450 2C9 overexpression in human hepatoma cells. Chem. Res. Toxicol. 22, 779-87 (2009). IHC, WB, Human 19445531

  Sookoian, S; Castaño, GO; Scian, R; San Martino, J; Pirola, CJ; Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH). 6, 22528 (2016). IHC, WB, Human 26935030

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: HSPB1 antibody tested with Human Hepg2 Cells (ARP30177_T100)

Product Protocols: HSPB1 antibody tested by IHC with human kidney (ARP30177)

Product Protocol: HSPB1 Antibody (ARP30177_T100) in Human Heart Muscle Tissue using Immunohistochemistry