HSPA1A Antibody (OAGA00883)

Catalog No: OAGA00883

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
B: NIH-3T3
C: Mouse brain
D: PC-12
E: Rat brain
7.5% SDS PAGE
diluted at 1:10000

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
7.5% SDS PAGE
OAGA00883 diluted at 1:10000

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
OAGA00883 diluted at 1:10000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00883 diluted at 1:10000

HSP70 1A antibody detects HSPA1A protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
HSP70 1A antibody (OAGA00883) dilution: 1:500.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using HSP70 1A(OAGA00883) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by 2.5 ?g of HSP70 1A antibody (OAGA00883) 12% SDS-PAGE The immunoprecipitated HSP70 1A protein was detected by HSP70 1A antibody (OAGA00883) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid 1XPBS, 20% glycerol (pH 7) with 0.01% Thimerosal added as a preservative
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, IP, WB
  Positive Controls: 293T, NIH-3T3, Mouse brain, PC-12, Rat brain, Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, Mesenchymal stem cells
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 308 and 569 of HSP70 1A
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Xenopus laevis (85%), Sheep (99%), Bovine (99%)
• Concentration: 0.73 mg/ml
• Application Info: ICC/IF: 1:100~1000
  IHC-FFPE: 1:100~1000
  IP: 1:500~1000
  WB: 1:5000~20000

Target Info

• Gene Symbol: HSPA1A
• Gene Full Name: Heat shock 70 kDa protein 1A/1B
• Alias Symbols: HSP72, HSPA1, HSP70I, HSP70-1, HSP70-2, HSP70.1, HSP70.2, HSP70-1A, HEL-S-103
• NCBI Gene Id: 3303
• Protein Name: Heat shock 70 kDa protein 1A/1B
• Description of Target: This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
• Uniprot ID: P08107
• Protein Accession #: NP_005336.3
• Molecular Weight: 70 kDa