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Datasheets/Manuals | Printable datasheet for OASE00148-FITC |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Monoclonal |
Clone | 1C4-1A6 |
Isotype | IgG1Kappa |
Host | Mouse |
Conjugation | FITC: Fluorescein Isothiocyanate |
Application | WB |
Additional Information | Background Info: Detects a 47kDa protein |
:: | Scientific Background: Hsp47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of Hsp47 around fibrotic lesions (1). The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since Hsp47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3). |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of Hsp47 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Human Hsp47, full length |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Dilution | 1:1000 (WB) |
Storage Buffer | PBS pH7.4, 50% glyercol and 0.09% sodium azide |
Reference | 1. Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212. 2. Taguchi T., et al. (2011) Acta Histochem Cytochem. 44(2):35-41. 3. Nong Z., et al. (2011) Am J Pathol. 197(5): 2189-96. |
Gene Symbol | SERPINH1 |
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Gene Full Name | serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) |
Alias Symbols | CBP1, CBP2, OI10, gp46, AsTP3, HSP47, PIG14, PPROM, RA-A47, SERPINH2 |
NCBI Gene Id | 871 |
Uniprot ID | P50454.2 |
Protein Accession # | NP_001193943 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Hsp47 Antibody : FITC (OASE00148-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Hsp47 Antibody : FITC (OASE00148-FITC)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "Hsp47 Antibody : FITC (OASE00148-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Hsp47 Antibody : FITC (OASE00148-FITC)"?
This target may also be called "CBP1, CBP2, OI10, gp46, AsTP3, HSP47, PIG14, PPROM, RA-A47, SERPINH2" in publications.
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What is the shipping cost for "Hsp47 Antibody : FITC (OASE00148-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Hsp47 Antibody : FITC (OASE00148-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Hsp47 Antibody : FITC (OASE00148-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Hsp47 Antibody : FITC (OASE00148-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SERPINH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SERPINH1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SERPINH1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SERPINH1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SERPINH1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SERPINH1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.