Hsp47 Antibody (OASE00148)

Catalog No: OASE00148

Size:200ug

Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57. Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57-47. Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide
• Clonality: Monoclonal
• Clone: 1C4-1A6
• Isotype: IgG1 Kappa
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, ICC, IF
  Cellular Localization: Endoplasmic reticulum lumen
  Certificate of Analysis: 1 ug/ml of SMC-203 was sufficient for detection of HSP47 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Human HSP47, full length
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects 47kDa.
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application Info: WB: 1:1000
  ICC/IF: 1:100
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212.
  2. Taguchi T., et al. (2011) Acta Histochem Cytochem. 44(2):35-41.
  3. Nong Z., et al. (2011) Am J Pathol. 197(5): 2189-96.
  
• Description: HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions (1). The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).

Target Info

• Gene Symbol: HSP47
• Gene Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Alias Symbols: SerpinH1, Colligin, Gp46, serine proteinase inhibitor, cysteine proteinase inhibitor, collagen binding protein
• NCBI Gene Id: 871
• Description of Target: HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions. The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate. Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils.
• Uniprot ID: P50454.2
• Protein Accession #: NP_001193943