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Catalog No: OKEH03077
Size:96T
Price: $525.00
SKU
OKEH03077
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH03077
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology SERPINH1 ELISA Kit (Mouse) (OKEH03077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Serpinh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinh1 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 9.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Serpinh1 Microplate96 Wells (12 x 8 Well strips)
Serpinh1 Lyophilized Standard2
100X Biotinylated Serpinh1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40.1 pg/mL
SpecificityNatural and recombinant Mouse Serpin H1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSerpinh1
Gene Full Nameserine (or cysteine) peptidase inhibitor, clade H, member 1
Alias Symbols47 kDa heat shock protein;BERF-1;beta enolase repressor factor 1;Cbp;Cbp1;Cbp2;co;collagen binding protein 1;Collagen-binding protein;colligin;gp4;gp46;Hsp;Hsp47;J6;Ser;serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1;serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2;serine protease inhibitor J6;serpin H1;Serpinh2.
NCBI Gene Id12406
Protein NameP19324
Description of TargetSerpin H1
Uniprot IDhttps://www.uniprot.org/uniprot/P19324
Protein Accession #NP_001104513.1
Nucleotide Accession #NM_001111043.1
Protein Size (# AA)417
Molecular Weight47 kDa
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