Hsp25/Hsp27 Antibody : Biotin (OASE00024-Biotin)

Catalog No: OASE00024-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat, Bovine, Canine, Guinea pig, Hamster
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 8A7
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IF, ICC, IP, IHC
  Certificate of Analysis: A 1:5000 dilution was sufficient for detection of hsp27 in 20ug of HeLa cell lysate by ECL immunoblot analysis.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Hsp27 peptide
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects an 25kDa or 27kDa protein corresponding to the molecular mass of Hsp25/27 on SDS PAGE immunoblots. Recognizes Hsp25 and Hsp27, cross reacts with alpha B crystallin.
• Dilution: WB 1:5000 (ECL), 1:200 (IF)
• Reference: 1. Welch W.J. (1985) J Biol. Chem. 260: 3058-3062. 2.Kim K.K., Kim R., and Kim S. (1998) Nature 394(6693): 595-599.
  3. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56.
  4. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229.
  5. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70.
  6. Welsh M.J., Wu W., Parvinem M., and Gilmont R.R. (1996) Biol. Of Reprod. 55: 141-151.
  7. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26.
  8. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.
  9. Jia, Y. et al. (2001) J. Biol. Chem. 276(43):39911-39918.

Target Info

• Gene Symbol: HSPB1
• Gene Full Name: heat shock 27kDa protein 1
• Alias Symbols: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102
• NCBI Gene Id: 3315
• Description of Target: Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction.
  Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).
• Uniprot ID: P04792
• Protein Accession #: NP_001532.1
• Molecular Weight: 25 or 27 kD