HDAC6 Antibody (Phospho-Ser22) (OASG03303)

Catalog No: OASG03303

Size:100 uL

Western Blot using 3T3

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF
  Cellular Localization: Nucleus. Cytoplasm. Perikaryon Cell projection, dendrite Cell projection, axon. It is mainly cytoplasmic, where it is associated with microtubules.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human HDAC6 around the phosphorylation site of S22. Location: 1-80aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phosphorylated by AURKA. Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.
• Specificity: Phospho-HDAC6 (S22) Polyclonal Antibody detects endogenous levels of HDAC6 protein only when phosphorylated at S22.
• Application Info: WB: 1:500~2000
  IHC: 1:100~300
  IF: 1:200~1000
  ELISA: 1:10000

Target Info

• Gene Symbol: HDAC6
• Gene Full Name: histone deacetylase 6
• Alias Symbols: HD6, JM21, CPBHM, PPP1R90
• NCBI Gene Id: 10013; 15185;
• Description of Target: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes . Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.
• Uniprot ID: Q9UBN7, Q9Z2V5
• Molecular Weight: 131 kDa