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Catalog No: OKIA00095 (Formerly GWB-CC643B)
Size:1x96WELLPLATE
Price: $509.00
SKU
OKIA00095
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration40 Minutes
ELISA Kit PrincipleAviva Systems Biology Haptoglobin ELISA Kit (Mouse) (OKIA00095) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well.
ELISA Kit Range1.95 ng/ml - 125 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Mouse Haptoglobin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Mouse Haptoglobin Calibrator1
Additional InformationIntended Use: The Haptoglobin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Haptoglobin in serum or plasma of mice.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.586 ng/ml
Predicted Homology Based on Immunogen SequenceMouse
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHP
Gene Full Namehaptoglobin
Alias Symbolszo, HP-, pre, HP-1, preHP2
NCBI Gene Id15439
Protein NameHaptoglobin
Description of TargetAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Uniprot IDQ61646
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