HAMP ELISA Kit (Dog) (OKCA02210)

Catalog No: OKCA02210

Size:96 Wells

Dog HAMP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	107	96-111
  1:2	100	90-104
  1:4	97	90-101
  1:8	91	82-95

• ELISA Kit Principle: Aviva Systems Biology HAMP ELISA Kit (Dog) (OKCA02210) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HAMP has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HAMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard HAMP captured in the well.
• ELISA Kit Range: 0.625 - 41 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	91	84-95
  EDTA Plasma (n=4)	107	99-111

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • HAMP Microplate: 96 Wells (12 x 8 Well strips)
  • HAMP Lyophilized Standard: 2
  • 100X Biotinylated HAMP Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HAMP
• Alias Symbols: HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide, putative liver tumor regressor
• NCBI Gene Id: 492281
• Protein Name: Hepcidin
• Description of Target: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity.
• Uniprot ID: Q5U9D2
• Protein Accession #: NP_001007141
• Nucleotide Accession #: NM_001007140