Catalog No: OKEH01408
Size:96 Wells
Price: $650.00
SKU
OKEH01408
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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HAMP ELISA Kit (Mouse) : 96 Wells (OKEH01408)

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01408
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HAMP ELISA Kit (Mouse) (OKEH01408) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HAMP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HAMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HAMP captured in well.
ELISA Kit Range6.25 - 400pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 11.7% (n = 20)
ELISA Kit Component
ComponentAmount
HAMP Microplate96 Wells (12 x 8 Well strips)
HAMP Lyophilized Standard2
100X Biotinylated HAMP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity3.165 pg/mL
SpecificityNatural and recombinant Mouse Hepcidin
Publications

A role of PIEZO1 in iron metabolism in mice and humans. Cell. 184, 969-982.e13 (2021). 33571427

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHAMP
Gene Full Namehepcidin antimicrobial peptide
Alias SymbolsHep, Hepc, Hamp1, Hepc1
NCBI Gene Id84506
Protein NameHepcidin
Swissprot IdQ9EQ21
Protein Accession #NP_115930.1
Nucleotide Accession #NM_032541.1
Protein Size (# AA)83
Molecular Weight9 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express HAMP.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express HAMP.
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