GLS ELISA Kit (Rat) (OKCA02231)

Catalog No: OKCA02231

Size:96 Wells

Rat GLS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	95	91-99
  Serum (n=4)	1:2	103	98-106
  Serum (n=4)	1:4	110	106-114
  Serum (n=4)	1:8	92	89-97

• ELISA Kit Principle: Aviva Systems Biology Gls ELISA Kit (Rat) (OKCA02231) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLS has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GLS captured in well.
• ELISA Kit Range: 25 pg/mL-1,600 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	84	81-89
  EDTA plasma (n=4)	97	93-100

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-GLS Microplate: 96 Wells (12 x 8 Well strips)
  • GLS Lyophilized Standard: 2 x 1.6 ng
  • 100X Biotinylated GLS Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 6.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GLS
• Alias Symbols: Glut, RATGLUT
• NCBI Gene Id: 24398
• Protein Name: Glutaminase kidney isoform, mitochondrial
• Description of Target: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.
• Uniprot ID: P13264
• Protein Accession #: NP_001103438
• Nucleotide Accession #: NM_001109968