GLS Antibody - C-terminal region (OAAB05406)

Catalog No: OAAB05406

Size:200UL

Western blot analysis of GLS Antibody (C-term) (Cat. #OAAB05406) in mouse liver tissue lysates (35ug/lane). GLS (arrow) was detected using the purified Pab.

GLS Antibody (C-term) (Cat. #OAAB05406) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of GLS Antibody (C-term) (Cat. #OAAB05406) with Hela cell followed by Alexa Fluor®489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

GLS Antibody (C-term) (Cat. #OAAB05406) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IF,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IF: 1:25
  IHC-P: 1:25
  FC: 1:25
  WB: 1:2000
• Other Applications Image 1 Data: GLS Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: GLS
• Alias Symbols: 6330442B14, AAD20, AI314027, B230365M23Rik, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, Glut, glutaminase C, glutaminase kidney isoform, mitochondrial, glutaminase, phosphate-activated, K-glutaminase, KGA, kidney-type glutaminase, L-glutamine amidohydrolase, RATGLUT.
• NCBI Gene Id: 14660|24398|2744
• Protein Name: Glutaminase kidney isoform, mitochondrial|glutaminase kidney isoform, mitochondrial isoform 1 precursor [Homo sapiens]|glutaminase kidney isoform, mitochondrial isoform 2 [Homo sapiens]
• Description of Target: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.|Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
• Uniprot ID: D3Z7P3|O94925|P13264
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001243239.1,NP_055720.3
• Molecular Weight: 73461