GABRB3 Antibody (Bi-Phospho-Ser408/409) (OAPC00162)

Catalog No: OAPC00162

Size:100 ul

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k GABAA beta 3 protein phosphorylated at Ser408/409 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Xenopus, Bovine, Chicken, Zebrafish, Canine
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For long term storage -80C is recommended, but shorter term storage at -20C is also acceptable as aliquots may be taken without freeze/thawing due the presence of 50% glycerol. Stock solution are stable for minimum of 1 year at -20C.
• Immunogen: The antigen is a phosphopeptide corresponding to amino acid residues surrounding the di-phospho-Ser408/409 of the rat GABAA receptor, beta-3 subunit.
• Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Concentration: 0.25 mg/ml
• Specificity: Specific for the ~53kD GABRB3 phosphorylated at Ser408/Ser 409. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
• Application Info: WB: 1:1000
• Reference: Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
  Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdelles B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653
  Ogris W, Poltl A, Hauer B, Ernst M, Oberto A, Wulff P, Hoger H, Wisden W, Sieghart W (2004)
  Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA
  receptor gamma 2-subunit. Biochem Pharmacol 68:1621-1629.
  Brandon NJ, Delmas P, Kittler JT, McDonald BJ, Sieghart W, Brown DA, Smart TG, Moss SJ (2000) GABAA receptor phosphorylation and functional modulation in cortical neurons by a protein kinase C-dependent pathway. J Biol Chem 275, 38856-62

Target Info

• Gene Symbol: GABRB3
• NCBI Gene Id: 24922
• Protein Name: Gamma-aminobutyric acid receptor subunit beta-3
• Description of Target: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the beta 3 subunit have been shown to be critical for the functional modulation of beta 3 containing recombinant receptors (Brandon et al., 2000).
• Uniprot ID: P63079
• Protein Accession #: NP_058761.1
• Molecular Weight: ~53kDa