EFNA5 Antibody (OAAF02312)

Catalog No: OAAF02312 (Formerly GWB-ASD555)

Size:100 ug

Western blot analysis of extracts from HeLa cells, using EFNA5 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EFNA5 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using EFNA5 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human EFNA5.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: EFNA5 antibody detects endogenous levels of total EFNA5 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000
• Reference: Winslow J.W., Neuron 14:973-981(1995).
  Kozlosky C.J., Cytokine 9:540-549(1997).
  Davy A., Genes Dev. 13:3125-3135(1999).

Target Info

• Gene Symbol: EFNA5
• Gene Full Name: ephrin A5
• Alias Symbols: AF1;AL-1;EFL5;eph-related receptor tyrosine kinase ligand 7;ephrin-A5;EPLG7;GLC1M;LERK7;LERK-7;RAGS.
• NCBI Gene Id: 1946
• Protein Name: Ephrin-A5
• Description of Target: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
• Uniprot ID: P52803
• Protein Accession #: NP_001953.1
• Molecular Weight: 26 kDa