Catalog No: OKCD01803 (Formerly GWB-KBBLM9)
Size:96WELLS
Price: $675.00
SKU
OKCD01803
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Datasheets/ManualsPrintable datasheet for EFNA5 ELISA Kit (Human) (OKCD01803)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)82-93%79-99%94-102%93-104%
EDTA Plasma (n=5)98-105%91-98%90-97%93-101%
Heparin Plasma (n=5)83-99%82-97%87-95%83-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin A5 (EFNA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin A5 (EFNA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin A5 (EFNA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ephrin A5 (EFNA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)79-9785
Heparin Plasma (n=5)79-10489
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin A5 (EFNA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EFNA5 Microplate96 Wells (12 x 8 Well Strips)
EFNA5 Lyophilized Standard2 x 10 ng
100X Biotinylated EFNA5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ephrin A5 (EFNA5). No significant cross-reactivity or interference between Ephrin A5 (EFNA5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEFNA5
Gene Full Nameephrin A5
Alias SymbolsAF1;AL-1;EFL5;eph-related receptor tyrosine kinase ligand 7;ephrin-A5;EPLG7;GLC1M;LERK7;LERK-7;RAGS.
NCBI Gene Id1946
Protein NameEphrin-A5
Description of TargetCell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
Uniprot IDP52803
Protein Accession #NP_001953.1
Nucleotide Accession #NM_001962.2
Protein Size (# AA)228
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